Effects of various agitation techniques on sealer penetration in dentinal tubules using CLSM

Aim: To compare the effect of ultrasonic, sonic and rotary sealer activation techniques on depth penetration in dentinal tubules. Materials Methods: Freshly extracted 60 single rooted human permanent mandibuar premolar with mature apex were collected. A size 10 no. K file (Dentsply Maillefer) was introduced into each canal. The irrigation procedure accomplished by using 2ml 5.25% NAOCl for used. Roots randomly divided 3 groups basis techniques. specimens kept incubator at 37 degree Centigrade temperature 2 days. dentin segments examined a confocal microscope Jawaharlal Nehru University, New Delhi under 4X,10X magnification. Results: total teeth included study they three method agitation sealer: GROUP I - Lentulospiral, Group II-Ultrasonic endodontic tip, III Endoactivator obturation done gutta percha. Mean percentage greatest II i.e. Ultrasonics when used statistically significant than lentulospiral Endoactivator.